rhythm Mitosync

Researchers investigated the absorption of oral L-BAIBA (MitoBurn®) in 12 healthy adults, comparing doses of 250 mg, 500 mg and 1,500 mg with valine and placebo.

What did they find?

All three L-BAIBA doses produced significantly higher plasma L-BAIBA levels than valine or placebo throughout the five hour assessment period.

• Direct absorption: Oral L-BAIBA was detected in the bloodstream following supplementation.
• Dose relevance: Absorption was demonstrated at 250 mg, the dose used in Mitosync.
• Comparison with valine: L-BAIBA supplementation produced higher plasma L-BAIBA levels than valine, its amino acid precursor, under the study conditions.

These findings confirm that oral L-BAIBA can increase circulating L-BAIBA levels in humans, including at the 250 mg dose studied.

Study details: Randomised, double blind, placebo controlled crossover trial · 12 healthy adults, 6 men and 6 women · 250 mg, 500 mg and 1,500 mg L-BAIBA compared with 1,500 mg valine and placebo · Blood sampling over 300 minutes.

Researchers investigated the effects of daily L-BAIBA supplementation in 37 overweight adults following a structured exercise programme and calorie controlled diet over 56 days.

What did they find?

Participants taking 500 mg of L-BAIBA daily reported lower hunger ratings than the placebo group at days 14, 28 and 56.

• Hunger: Lower hunger ratings were reported at all three assessment points.
• Consistency: The difference was observed throughout the 8 week study.
• Lifestyle support: Supplementation was studied alongside a structured exercise programme and calorie controlled diet.

These findings suggest that L-BAIBA may influence subjective hunger during a structured weight management programme.

Study details: Randomised, double blind, placebo controlled trial · 37 overweight adults · 500 mg L-BAIBA (MitoBurn®) daily for 8 weeks · Hunger, appetite and body composition assessed at weeks 0, 2, 4 and 8.

Researchers investigated changes in circulating L-BAIBA during aerobic exercise in healthy adults, measuring plasma levels of R and S - BAIBA using high performance liquid chromatography (HPLC).

What did they find?

A single session of aerobic exercise increased plasma L-BAIBA levels by approximately 20%.

• Exercise response: L-BAIBA levels increased following aerobic exercise.
• Natural signalling molecule: L-BAIBA is produced by the body and can be released into circulation during exercise.
• Relevance to Mitosync: Mitosync provides L-BAIBA directly through supplementation, rather than relying solely on the body's own production.

These findings show that L-BAIBA levels can rise during exercise.

Study details: Human exercise trial · Healthy adults · Single bout of aerobic exercise · Plasma R and S-BAIBA measured using HPLC.

Researchers investigated the effects of L-BAIBA on bone cells exposed to oxidative stress and in a mouse model of disuse, examining mitochondrial integrity, bone loss and muscle function.

What did they find?

L-BAIBA prevented oxidative stress induced mitochondrial breakdown in bone cells. In the mouse model, it also helped prevent bone loss and loss of muscle function.

• Mitochondrial protection: Helped protect mitochondria in bone cells exposed to oxidative stress.
• Cellular resilience: Showed protective effects comparable to estrogen or N-acetyl cysteine in the experimental model.
• Muscle function: Helped preserve muscle function in mice subjected to disuse.

These findings suggest that L-BAIBA may have biological effects beyond metabolism.

Study details: Preclinical study · Bone cell experiments and mouse hindlimb unloading model · L-BAIBA administered in drinking water in the animal study.

Researchers investigated the effects of L-BAIBA in skeletal muscle cells and mice fed a high fat diet, examining AMPK activation, PPARδ expression and metabolic signalling.

What did they find?

L-BAIBA significantly activated AMPK, a key regulator of cellular energy metabolism, and increased PPARδ expression in skeletal muscle.

• Energy signalling: Activated AMPK, a pathway involved in cellular energy regulation.
• Fat oxidation: Findings supported a role for the AMPK–PPARδ pathway in regulating fat metabolism in muscle.
• Metabolic health: The study reported effects on insulin signaling and inflammation in the experimental models.

These findings provide mechanistic insight into how L-BAIBA may influence muscle energy metabolism.

Study details: Preclinical study · Mouse skeletal muscle cells and high fat diet mice · Molecular signaling analysis.

Researchers investigated L-BAIBA as a small molecule associated with muscle activity, examining its role in fat metabolism using cell and animal models, alongside human cohort data.

What did they find?

• Fat metabolism: L-BAIBA increased fat oxidation in liver cells.
• Fat cell activity: L-BAIBA activated genes associated with brown fat characteristics in white fat cells.
• Glucose handling: Improved glucose handling was observed in mice.
• Human observations: Circulating L-BAIBA levels increased with exercise and were inversely associated with metabolic risk factors.

These findings highlight L-BAIBA as a potential link between exercise and metabolic regulation.

Study details: Preclinical cell and animal study with human cohort data · Metabolomic identification of muscle secreted factors.

Researchers investigated the role of L-BAIBA in muscle adaptation, combining mouse supplementation and muscle knockdown models with human data on aerobic fitness and endurance training.

What did they find?

In mice, L-BAIBA supplementation increased the number and capacity of muscle mitochondria and improved muscle contraction. The changes resembled adaptations associated with aerobic exercise training.

• Mitochondrial function: Increased mitochondrial number and capacity in mouse muscle.
• Muscle performance: Improved muscle contraction in the experimental model.
• Human exercise data: Plasma L-BAIBA levels were associated with aerobic fitness and increased with endurance training.

These findings highlight L-BAIBA's potential role in muscle adaptation and energy metabolism.

Study details: Preclinical study with human plasma data · Mouse supplementation and muscle knockdown models · Human aerobic fitness and endurance training cohorts · Mitochondrial, metabolic and contractile function.

Researchers evaluated the safety of daily oral L-BAIBA supplementation in rats over 90 days, testing doses of 100, 300 and 900 mg/kg body weight per day.

What did they find?

The study established a no observed adverse effect level (NOAEL) of 900 mg/kg body weight per day, the highest dose tested.

• Repeated dose safety: No adverse effects were observed at the highest dose tested.
• Dose evaluation: Three dose levels were assessed over 90 consecutive days.
• Safety assessment: The findings provide a substantial body of repeated dose toxicology data for L-BAIBA.

Regulatory status: MitoBurn® has self affirmed Generally Recognised as Safe (GRAS) status for use in sports drinks, nutrition bars and powdered beverages, supporting its intended.

Study details: Preclinical safety study · 100 Sprague Dawley rats, including main and recovery groups · Oral L-BAIBA at 100, 300 and 900 mg/kg/day for 90 days · Subchronic repeated dose toxicity assessment.